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Jitendra bajaj1, *Vivek Dave2, Swapnil Sharma2, Ajay Shukla3, R.D. chakole4

1Department of Pharmacy, NRI Institute of Pharmacy, Bhopal, M.P., India
2Department of Pharmacy, Banasthali University, Banasthali, Raj., India.
3Department of Pharmacy, G. I.T, Jabalpur, M.P., India
4Department of Pharmacy, Govt. Polytechnic College, Amravati, Maharashtra, India


The powder of aerial parts of Achyranthes aspera was screened for pharmacognostic character, fluorescence analysis and histochemical analysis. This powder was also processed for determination of qualitative and quantitative of some phytochemical constituents. Pharmacognostical studies comprised of taxonomical characters, macro and microscopical characters of the part used histochemical tests and diagnostic characters were performed. Physicochemical constants such as ash, extractive values and moisture content were determined. The fluorescence analysis was done by treating moderately coarse powder with different reagents and observed under visible and ultra violet light (short wavelength and long wavelength). Quantitative and qualitative phytochemical analyses were also performed to confirm active constituent in the extract. The physiochemical analysis showed that the aerial part of this plant contained ash values 10 % in which acid-insoluble ash is 1.49% and water soluble is 6.5% moisture content is 200 mg/100 gm. The extracts were subjected to qualitative screening test for various constituents. This revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids and steroids. Quantitative analysis is also performed in methanolic extracts in which total glycosides, total alkaloid, total saponin and total flavonoids were found to be 9%, 2% , 0.4% 1% respectively.

Keywords: Achyranthes aspera, methanolic extract, qualitative screening, quantitative analysis.

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