Abstract

TO STUDY THE HEMATOLOGICAL AND LIPID PROFILE ALTERATIONS AFTER CO-EXPOSURE OF NICOTINE AND CHROMIUM (VI) IN MALE ALBINO RAT

Soumita Dey, Amit Nandi, Monalisha Paul, Sandip Kumar Sinha and Sankar Kumar Dey*

ABSTRACT

The significant numbers of people continue to smoke in the developing countries. Prevalence of smoking in the population gradually reached day to day. Smoking is a known risk factor for respiratory, cardiovascular, neoplastic and other diseases in our community. It was reported that elevation of some trace element such as chromium and cobalt was observed high in smoker as compared to non-smoker. Chromium in tobacco smoke is public health concern because of their potential toxicity and carcinogenicity. In this study, we evaluate the effects of individual and combined exposure of nicotine and chromium (VI) on hematological and lipid profile alterations in animal. In this investigation, a group of male Wistar rats (80-100 g) were induced by intraperitonial injection of vehicle (0.9% NaCl), nicotine tartrate (0.2 mg / 100 g body weight / day), K2Cr2O7 (0.8 mg / 100 g body weight / day) and, combined exposure of nicotine tartrate and K2Cr2O7 at an interval of six hours for a period of 28 days. Results showed that individual and combined exposure of nicotine and chromium (VI) marked changes the levels of total erythrocyte count (TEC), hemoglobin (Hb), PCV, MCV, MCH, MCHC, total leukocyte count (TLC) and the percentage of neutrophils, lymphocytes, monocytes, eosinophils and basophils. On the other hand, marked alterations were observed in the levels of triglyceride, total cholesterol, LDL-cholesterol, HDL-cholesterol and VLDL- cholesterol in response to individual and combined exposure of nicotine and chromium. The present study suggests that nicotine and chromium exhibited significant changes during individual exposure whereas co-exposure showed variable conditions, indicative of both synergism and antagonism in male rats.

Keywords: Nicotine chromium co-exposure, Hematological alterations, Lipid profile status.