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Abstract

DEVELOPMENT AND VALIDATION OF COIXOL IN ETHANOLIC SEED EXTRACT OF Coix Lacryma Jobi L.

Dr. Bhavna Patel, Gopi Patel* and Dr. Samir Shah

ABSTRACT

A UV- Visible Spectrophotometric method has been developed for the determination of Coixol in ethanolic seed extract of Coix Lacryma Jobi L. The spectrophotometric detection was carried out at an absorption maximum of 286 nm using methanol as solvent. The method was validated for various parameters according to ICH Q2 (R1) guideline. The detector response for the Coixol in ethanolic seed extract was linear over the selected concentration range 10 to 50 μg/ml with a correlation coefficient of 0.9910. The accuracy was between 99.96 % & 100.08 %. The LOD and LOQ are 2.48 and 7.54 μg/ml respectively. The developed method was precise and accurate.

Keywords: Lacryma jobi L., Coixol, Extract, Validation, ICH guideline.


[Full Text Article]

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