Abstract

CYTOTOXICITY OF RAMIE (Boehmeria nivea L. Gaud.) LEAVES EXTRACT ON MCF-7 BREAST CANCER CELL LINE

Annisa Abdiwijaya Qaromah*, Asri Peni Wulandari, Desi Harneti

ABSTRACT

The objective of this study was to investigate the cytotoxicity effect of the crude extract and fractions of Ramie (Boehmeria nivea L. Gaud.) leaves extract on in vitro MCF-7 breast cancer cell line. In vitro cytotoxicity were evaluated by MTT-based assays. The percentage of cell inhibition was analyzed using probit analysis to obtain 50% inhibitory concentration (IC50). Morphological alteration of the cell line after exposure with extract were observed under inverted microscope. The cytotoxicity assay revealed that the ethanol extract and the methylene chloride fraction from the ramie leaves exhibited marked anticancer activity and has strong antiproliferative with IC50 of 3.27 μg/mL and 3.79 μg/mL, respectively, on MCF-7 cancer cell line. Morphological alteration of the cell line after exposure with Boehmeria nivea L. Gaud. extract were observed under inverted microscope in the
dose dependent manner. The crude extract and fractions of the Ramie leaves exhibit cytotoxic activity against MCF-7 cell line and showed morphological changes when incubated with the extract. Boehmeria nivea L. Gaud. could be considered as a potential source of the anticancer agent. However, further research to determine the exact mechanism of action needs to be carried out.

Keywords: Cytotoxicity, Boehmeria nivea L. Gaud., fractions, MTT assay.