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Moetazza M. Alshafei*, Emtenan M. Hanafi, Seham S. Kassem and Manal M. Ramadan


Osteoporosis and elevation of both blood cholesterol and low-density lipoprotein are an inevitable results of menopause in women as well as in overiectomized rats, both problems can be complicated by multiple health hazards. Objective: This study aimed to evaluate the effect of Lepidium sativum seeds on both problems in overiectomized rats. Material and method: Phenolic profile of LS was analyzed by HPLC. Thirty Sprague-dawely adult female rats will be divided into two groups G1 (N 10) control group (NOVXC) (sham incision), G2 (N 20) (COVX) overiectomized rats. Both groups will receive basic diet for 4weeks at which blood sample were collected. G2 will be divided into two groups one,treatment group, which will receive 20% of the basic diet as Lepidium sativum (OVX LS) (N10) for 8 weeks,the other will continue on basic diet (COVX). At the end of the experiment period, animals were fasted over night and sacrificed. Blood samples were collected for analysis of cholesterol, serum low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides TG, Malondialdhyde (MDA) Zinc, phosphrous, calcium and bone formation marker( PINP).Dual x ray Absorpiometry (Dexa) will be done for all overiectomized rats before and after treatment. Results: Dexa showed that group of overiectomized treated rats OVXLS have significant improvement of bone mineral density with elevation of Mg, zinc and PINP versus COVX. Lipid profile showed significant lowering of cholesterol and LDL as well as decrease of lipid peroxidation marker MAD in treatment group (P ≤ 0.05). Conclusion: LS seeds improved bone density and controlled elevated lipids in blood of overiectomized rats, treatment may has valuable action in osteoporosis and dyslipidemia due to menopause.

Keywords: Lepidium Sativum, overiectomy, rats, osteoporosis, hyperlipidemia.

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