Abstract

ANTIOXIDANT ACTIVITY OF THE FLOWER FRUIT AND LEAF, ANITDESMA VELUTINUM (TULAS) – A COMPARISON

Supachai Chumchuen*

ABSTRACT

The point of this research is to compare some parts (flowers fruits and leaves) of Antidesma velutinum extracted, antioxidant activity, total phenolic content and total flavonoid content of ethanol by soxhlet method. Correlation was considered by statistical analysis. The result showed, The highest of percent yield found in leaf 29.13 ± 0.01 and total phenolic and flavonoid contents relates with this have 87.65 ± 0.18 mg GAE/100 mg and 10.45 ± 0.01 RE/100 mg respectively. The comparison of phenolic and flavonoid contents of Antidesma velutinum, in each part was significantly different p < 0.01. For antioxidant activities, DPPH scavenging activity assay have IC50 of leaf was 13.96± 0.29μg/ml, the higest. white positive control, BHT presented higher activity with IC50 9.08 ± 0.74μg/ml. The ABTS radical cation showed higher at BHT with IC50 76.4 ± 2.44μg/ml, the IC50 of ABTS radical cation of leaf the higest all of the organs, 69.4 ± 5.98μg/ml. The methods (DPPH and ABTS) for detected has a high correlation with the total phenolic analyzed by DPPH and ABTS, r = 0.831 and 0.793 respectively. However, the development product of this seems to be an interesting for further studies.

Keywords: antidesma velutinum, antioxidant activity, thai traditional medicine, total phenolic, total flavonoid.