Abstract

STUDY OF ANTI-ADHESIVE ACTION OF BIOSURFCATANTS EXTRACTED FROM Lactobacillus spp. on CAUTI CAUSING ORGANISMS

Shilpa Sabnis* and Sayali Kale

ABSTRACT

Biosurfactants effectively inhibit bacterial adhesion and retard biofilm formation and therefore have been reported as a promising strategy for development of potentially useful new generation anti-adhesive and antimicrobial coatings for medical devices. In the present study, biosurfactant producer strain isolated from cabbage samples, on characterization, was found to belong to Lactobacillus spp., as confirmed by 16S rRNA sequencing. The biosurfactant produced by the isolates was extracted using Acetone Precipitation Method. The anti-adhesive activity of the extracted biosurfactants against two catheter associated urinary tract infection (CAUTI) causing pathogens viz. E.coli and Candida albicans was studied qualitatively by Monochrome staining and quantitatively by Tube method and Blot Succession Technique and confirmed by Scanning Electron Microscopy.

Keywords: Biosurfactant, Lactobacilli, anti-adhesive, cauti.