Abstract

COMPARISION OF DERMAL WOUNDN HEALING ACTIVITY OF MANJISHTA EXTRACT IMPREGNATED COLLAGEN AND CROSS LINKED COLLAGEN DERMAL FILMS

M.Kishore Babu*, D.Mohan Krishna, V.Kalyani, P.V.Nagarani, D.V.D.Murthy

ABSTRACT

The aim of this study is to hasten and to improve the wound healing quality. Ensuring the physicochemical compatibility, Manjistha Extract impregnated Collagen & crosslinked Collagen dermal films (MEICDF & MEICCDF) were formulated using different concentrations such as 10%w/v, 15%w/v and 20%w/v of Manjishta. The formulated films were subjected to physical, biochemical and histopathological examinations. Wound healing studies on Male Wister Rats were performed for a period of 7 days and it was observed that the 15%w/v of both MEICDF & MEICCDF treated rats possessed higher amount of Hydroxy Proline content 70.2% & 71.0% when compared to that of the existing Marketed formulation (Neu- skinTM) (60.59%). Further, better wound healing activity was observed in the 15%w/v MEICDF & MEICCDF treated rats (77.1% & 79.1%). When examined histopathologically, more production in the collagen content was clearly observed in 15% w/v MEICDF & MEICCDF treated groups which resulted in the epithelial gap reduction. The more count of fibroblast (64 & 65 in 100μ in the wound during the healing process) in 15% MEICDF & MEICCDF treated groups suggests that it is a feasible and productive approach in the improvement of dermal wound healing process.

Keywords: Collagen, Crosslinking; Manjishta Extract; Micro shrinkage;Temperature; Wound Healing; Histopathological Examination.