Abstract

ANTIOXIDANT ACTIVITY FROM ETHANOLIC EXTRACT OF DIATOM NITZSCHIA LONGISSIMA

M. Gopinath and P. Sampathkumar*

ABSTRACT

The present study is carried out to perform the in vitro total antioxidant assay, total phenolic content, DPPH assay, reducing power assay, nitric oxide scavenging activity and hydrogen peroxide scavenging activity of ethanolic extract from pennate diatom, Nitzschia longissima. The total phenolic content of Nitzschia longissima was 1.72 ± 0.13μg/ml and the total antioxidant assay was observed as 47.59 ± 0.40 %. The ethanolic extract of Nitzschia longissima showed 75.36 ± 1.90% in DPPH assay, reducing power assay of 77.81 ± 1.22%, nitric oxide scavenging activity of 61.46 ± 1.30% and the hydrogen peroxide scavenging activity of 80.67± 1.20%. Based on the activities assay conducted in the present study and revealed that Nitzschia longissima can be act as natural antioxidant source for food preservation and protection from degenerative diseases.

Keywords: Marine diatom, total antioxidant assay, total phenolic content, DPPH assay, reducing power assay, nitric oxide scavenging activity, hydrogen peroxide scavenging activity.