Abstract

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AERIAL PARTS OF BARLERIA MONTANA PLANT

Beena H. M.* and Dr. M. S. Rajesh

ABSTRACT

The present study was designed to evaluate the Hepatoprotective activity of aerial parts of Barleria Montana plant by inducing Paracetamol and INH + Rifampicin which causes hepatotoxicity in rats. The Aerial part of the plant Barleria Montana was collected, shade dried, powdered and extracted by using water and methanol in the ratio of 30:70 manually with the help of soxhlet extractor. Preliminary phytochemical investigation is carried out. Acute oral toxicity study was conducted as per OECD guidelines (420) in order to ascertain LD50 value of the extract. The hepatoprotective activity of hydro alcoholic extract of Barleria Montana was tested using two different models namely Paracetamol (7 days) and INH +Rifampicin (15days and 30days respectively) by measuring biomarkers like SGOT, SGPT, ALP, TB and TSP. The parameters like SGPT, SGOT, ALP and Total bilirubin (TB) levels are significantly increased in the positive control group of both the models as compared to normal. Pre-treatment with (200 and 400 mg/kg bw p. o.) has decreased the levels of SGPT, SGOT, ALP and TB significantly as compared to positive control group. Silymarin pre-treatment has also produced a significant decrease in SGPT, SGOT, ALP and TB when compared to positive control group. On the other hand positive control group has shown decreased levels of Total serum protein as compared to normal group. Pre-treatment with silymarin increased the TSP level and (200 and 400 mg/kg bw p. o.) restored the levels of TSP almost equivalent to normal rat values. The histopathology of rat liver was studied to find the hepatoprotection by the extract.

Keywords: Barleria Montana, Paracetamol, INH, Rifampicin, Silymarin.