Abstract

ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A REVIEW

Nutan Rao*, Shraddha Gaur, Akshata Desai and Madhusudan Bhoir

ABSTRACT

Ultra Performance Liquid Chromatography (UPLC) is derived from High Performance Liquid Chromatography (HPLC). UPLC principle is based on Van Deemter equation as that of HPLC. Advances in instrumentation and column technologies were made to achieve significant increase in sensitivity, speed and resolution in liquid chromatography which is now known as UPLC system. UPLC can show advantage of the separation performance (by lowering dead volumes) and consistent with the pressures. Main advantage of UPLC is that the chromatogram obtained gives resolved peaks, and well separated peaks. In UPLC system it follows small particle chemistry that is decrease in particle size has increased efficiency at higher flow rates. Smaller particles have the ability to work at higher linear velocity without loss of efficiency, also they provide high resolution and speed.

Keywords: UPLC, HPLC, Van Deemter Equation, Pump, Columns.