Abstract

IN-VITRO SCREENING FOR ACETYLCHOLINESTERASE ENZYME INHIBITION POTENTIAL AND ANTI-INFLAMMATORY ACTIVITY OF MALDEVI CHENDURAM: THERAPEUTIC LEAD FOR NEUROLOGICAL DISEASES

Ajantha R.*, Ishwarya K., Manikgantan E. M. and Kabilan N.

ABSTRACT

Chroic inflammation and increased expression of acetylcholinesterase (AChE) enzyme plays an vital role in hydrolysis of the most important cognitive neurotransmitter called Acetylcholine (ACh), decrease in ACh level leads to various neurological and degenratives disease. The main objective of this study is to evaluate acetylcholinesterase inhibition and anti- inflammatory activity of the siddha formulation Maldevi Chenduram (MC), which is a classical siddha medicine formulated by using metals and minerals. It was indicated for neurological disorders, depression and other psychiatric disorders as per the standard text. Though the formulation MC was effective in treating neurological disorder, scientific evaluation is necessary for validating its efficacy. The acetylcholinesterase enzyme inhibition potential was analysed based on Ellman’s method of modified 96-well microplate assay whereas anti-inflammatory activity was analysed using albumin protein denaturation assay. The results of the present study clealry indicates that the siddha drug MC possess significant acetylcholinesterase inhibition activity of about 67.97% was observed at 500 μg/ml with the IC value of 297.2 ± 23.42 μg/ml. And possess significant anti-inflammatory property with a maximum percentage inhibition of about 44.46 ± 3.2% was observed at 500μg/ml. It was concluded from the results that the formulation MC may be advocated for treating neurological problems in particular to Alzheimer’s and related dementia at clinical level with prior in-vivo validation.

Keywords: Acetylcholinesterase, Anti-inflammatory, Maldevi Chenduram, Neurological diseases, Siddha medicine.