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Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY AND GCMS ANALYSIS OF FLAVOPARMELIA CAPERATA [L.].

*Deshpande S. N., Agnihotri I. M., Akkalkote R. S. and Birajdar P. V.

 

ABSTRACT

Bacterial diseases are treated with antibiotics but resistance to antibiotic is a global problem. An alternative to this is plant medicine. The symbiotic association between fungi and algae called lichen has been used in folk medicine. The present study was therefore aimed at determining the antibacterial activity and phytochemical analysis of Flavoparmelia caperata [L.]. The extract of Flavoparmelia caperata [L.] were prepared by cold extraction method by using methanol and acetone as solvent. The antibacterial activity was determined by agar diffusion method while the phytochemical analysis was performed by chemical tests and by GCMS method. Preliminary phytochemical analysis showed the presence of carbohydrates in both acetone and methanol extract. Alkaloids and saponins were found present in methanol extract. Antibacterial activity was present in both the extracts. However maximum activity was found against Proteus mirabilis followed by Staphylococcus aureus. GCMS analysis revealed the presence 3-Methoxy-5-propylphenol, Benzoic acid,2,4-dihydroxy-3,6-dimethyl-, methyl ester, 9,12-Octadecadienoic acid (Z,Z)-, methyl ester,1,3-Benzenediol, 2,5-dimethyl- and Hexadecanoic acid, methyl ester in methanol extract, however, acetone extract showed the presence of 9-Tetradecen-1-ol, acetate, (E)-and followed by 3-Methoxy-5-propylphenol and 1,3-Benzenediol, 2,5-dimethyl.

Keywords: Lichen, agar diffusion, Flavoparmelia caperata [L.], GCMS, Alkaloids, Proteus mirabilis, Hexadecanoic acid.


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