Abstract

ANALGESIC AND ANTIOXIDANT EFFECTS OF LABLAB PURPUREUS LINN IN POLYPHENOLIC FRACTION AND METHANOLIC LEAF EXTRACT ON EXPERIMENTAL ANIMAL MODEL

C. Pramod, Prof. Dr. Sudhakaran Nair*, Prof. Dr. Jyoti Harindran and Prof. Dr. Ratheesh

ABSTRACT

Lablab purpureus Linn is a traditional plant widely used as a nutritional source. We conducted qualitative analyses by liquid chromatography-mass spectrometry and phytochemical evaluation of methanolic extract LPL. Analgesic activity was analyzed and results showed that the methanolic MEL extract had the highest activity at dose of 200mg/kg when compared with PPF at dose of 20mg/kg. The PPF of methanolic extract had the highest capacity to increased antioxidant enzyme like SOD, CAT, GPx and GSH during the estimation. The latency of pain from a hot-plate test was augmented by the methanolic extract when the dose was 200mg/kg compared with polyphenolic extract 20mg/kg. The PPF significantly decreased the amount of writhing in a dose-dependent manner compared with the control group in the acetic acid-induced writhing test. These results showed that LPL is an antioxidant and analgesic agent, and this study provides information on its ingredients for further study.

Keywords: Lablab purpureus Linn (LPL), PPF polyphenolic fraction, MEL Methanolic extract.