Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: JUNE ISSUE PUBLISHED
  • JUNE 2021 Issue has been successfully launched on 1 June 2021.

Abstract

STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF FLUTICASONE AND VILANTEROL IN BULK AND PHARMACEUTICAL FORMULATION

Aruna Gundala*, Hari Krishna Y. and Jayachandra Reddy P.

 

ABSTRACT

The present study was designed to develop and validate a simple, sensitive, precise and accurate stability indicating RP-HPLC method for simultaneous estimation of fluticasone and vilanterol in bulk and tablet dosage form. The chromatographic separation was achieved on Zodiac C18 column (250 x 4.6 mm, 5μm) as stationary phase with a mobile phase of water (PH 5.4 adjusted with ortho phosphoric acid):acetonitrile (60:40 v/v) at a flow rate of 1 ml/min and PDA detection at 255 nm. The proposed method was validated for system suitability, specificity, linearity, accuracy, precision, LOD, LOQ and robustness as per ICH guidelines. The retention times of fluticasone and vilanterol were found to be 2.528± 0.04 and 3.782± 0.07 min respectively. The calibration curves were linear in the concentration range of 50% to 150% of the working concentration (r2=0.999) for both the drugs in binary mixture. The accuracy was found to be 99.26% and 99.45 % for fluticasone and vilanterol respectively. The LOD was found to be 0.19μg/ml and 0.12μg/ml and LOQ was found to be 0.57 μg/ml and 0.36 μg/ml for fluticasone and vilanterol respectively. The percentage recoveries for both drugs were in the range of 99-100%. Hence the proposed stability indicating RP-HPLC method can be used in routine analysis of tablets containing fluticasone and vilanterol.

Keywords: Fluticasone, Vilanterol, RP-HPLC, stability, Method development and Validation.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More