Abstract

EFFECT OF ABIOTIC ELICITORS ON SOME SECONDARY METABOLITES PRODUCTION IN MELIA AZEDARACH L. IN VITRO

Alaa Jabbar Taha*

ABSTRACT

This research was conduct in the plant tissue culture laboratory, Biology Department, College of Science, Al-Mustansiriyah University. The major objective of this study was to increase production of some secondary metabolites using Silver nitrate as abiotic elicitors added to the culture medium of Melia azedarach L., and compare the productivity of this compound with those produced by the intact plants. Plant leaves were disinfected by NaOCl at 1% for 5 min. Callus was initiated from leaves explants cultured on MS medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) at concentrations 1.0 mg/l, the same culture medium was use for callus maintenance. The quality and quantity of phytochemicals were estimate using High performance Liquid Chromatography (HPLC), for methanol extracts of leaves sample and Callus. Results showed an increase of secondary metabolites concentration in methanol extracts of callus cultures compared with leaves extract. Callus cultures were treat with silver nitrate (AgNo3) at the concentrations 0.1, 0.2, 0.3 or 0.4 mM. Results showed all concentration of secondary metabolites were increased, P-hydroxy benzoic acid, Azadirachtin were record significant differences compared with control, reached 6160.0, 612000 mg\g respectively at 0.3 (Mm)). While p-cinamoylmelianolone, 1-cinnamoyl-3,11-dihydroxy meliacarpin and Salanin concentration reached, 0.6272, 0.1821 and 61..20 mg\g at 0.1,0.3 and 0.4 (Mm) respectively but not recorded significant differences. Results also showed that using silver nitrate caused decreasing of fresh and dry weight without any significant differences compared to control.

Keywords: In vitro, abiotic elicitors, Melia azedarach L., Secondary compounds.