Abstract

POTENTIAL ROLE OF GOLD NANOPARTICLES ON ULCERATED MODEL RATS: MOLECULAR STUDY

Rana Elsayed, Rokaya H. Shalaby*, Mostafa El-Sayed, Abdel- Rahman Zekri and Ola S. Ahmed

ABSTRACT

Gastric ulcers: disturbance in the gastric mucosa affect many people all over the world. It developed as an imbalance between the protective and aggressive factors. The objective of this study was to investigate the effect of gold nanoparticles (AuNPs) on treatment of peptic ulcers with using zinc as a conveying element to direct the nanogold particles to the affected sites. These treatments were carried out with different sizes of gold nanoparticles with green solid state laser doses. DNA fragmentation assay and the expression level of caspase-3, BAX and BCL-2 genes were evaluated in ulcerated gastric tissues using qRT-PCR. Our results indicated high level of DNA fragmentation in ulcerated group while this level was reduced after nanoparticles, zinc and laser exposure for 10 and 15 mins. Moreover, the use of gold nanoparticles with zinc and laser were significantly downregulated the expression levels of both caspase-3 and BAX gene as compared to ulcerated group, indicating a proapoptotic effect through caspase pathway. Moreover, significant increase in expression level of BCL-2 was detected as compared to ulcerated group indicated that gold nanoparticles exerted potent anti-apoptotic effect through BCL-2 pathway. Conclusion: The present data indicated potent gastro treatment effect by using gold nanoparticles with zinc (conveying element) and laser which also produced an inhibitory effect against ulceration via anti oxidative effect.

Keywords: Gold nanoparticles, Indomethacin, zinc, green solid state laser, DNA fragmentation, apoptotic genes.