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K. Lakshman*, Vinutha Bhat and V. R. Archana



Ethanolic extract of G. globosa leaves was subjected to investigate the antilithiatic activity by both in-vitro was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04–3 mg/mL) for 30 min. and in-vivo was evaluated by preventive and curative effect in ethylene glycol induced urolithiasis in rats. Wister rats were divided into VII groups, Ethylene glycol (0.75%)in drinking water was fed to all the groups (Group II-VII) except normal control (Group- I) for days to induced urolithiasis for curative and preventive regime. Group I, II and III served as normal control, positive control and standard (cystone 750 mg/kg). Group IV and V serves as curative and groups VI and VII as preventive were treated with ethanolic extract of G.globosa leaves respectively. Various biochemical parameters such as oxalate, calcium, phosphorus, urea creatinine, uric acid and blood urea nitrogen (BUN) levels were evaluated using urine and serum. histopathology of kidney were also recorded. Ethanolic extract of G. globosa inhibits calcium oxalate nucleation and aggregation and significantly resotre the biochemical to normal level. The histopathological examination also reverted after treatement with ethoanolic extract of G.globosa. The leaf extract of G.globosa reduces and inhibits the growth of urinary stones showing its effect as an antiurolithiatic agent

Keywords: Antilithiatic, calcium oxalate, nucleation, ethylene glycol, aggregration.

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