Abstract

ASSESSMENT OF CURCUMIN LOADED ALGINATE NANOBITES IN ACETIC ACID INDUCED COLITIS MODEL IN RATS

Harish K.H.*, Jayanthi C., Roopa G. and Hanumanthachar Joshi

ABSTRACT

Curcumin loaded sodium alginate polysorbate 80 nanoparticles prepared by ionotropic gelation method, successfully entrapped curcumin, exhibiting higher encapsulation efficiency nearing to 95%. Nanobites produced were in the size range 105-383 nm. FTIR Spectroscopy has shown that entrapped Curcumin in alginate polysorbate nanoparticles has interacted with Curcumin through hydrophobic interaction and ether linkage. DSC thermograms exhibited the conservation of crystalline status in the optimized formulation CA7. Curcumin released in both the media (SGF & SIF) was observed to be very low i.e less than 3%. SEM images of the nanobites revealed that they were less spherical, discrete and homogenous. Optimized formulation CA7 was subjected to in vivo studies in acetic acid induced colitis model in rats. The anti-inflammatory effect was evaluated by examining clinical parameters like change in body weights, presence / absence of blood in the stools and histopathological assessment of the colon tissue. It was found that the anti inflammatory effect of the Curcumin nanosuspension was more effective and greater when compared to the Curcumin suspension and the standard drug Meslamine. The study of In vitro bioadhesion revealed that all the prepared nanoparticles had good bioadhesive property ranging from 43% to 63%.

Keywords: Curcumin, Alginate, Nanobites, Acetic acid, Colitis, Rats, Mucoadhesion.