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Abstract

SPECTROPHOTOMETRIC DETERMINATION OF DRUGS BASED ON OXIDATION BY ACIDIC CHLORAMINE-T USING INDIGO CARAMINE DYE

Sailaja Battu, Venkateshwarlu Gandu* and Bhavani P. Nenavathu

 

ABSTRACT

The five drugs viz., Moxifloxacin (MOX), Dobutamine (DOB), Esmolol HCl (ESM), Voriconazole (VOR), Trimetazedine di HCL (TRM) were estimated using a simple, specific, accurate and precise UV–visible spectrophotometric method. These methods involve the addition of a known excess of Chloramine-T to the drugs in acid medium followed by estimation of residual Chloramine-T by reacting with a fixed amount of Indigo caramine dye and measuring the absorbance of the dye at 608 nm. The methods that are developed were found to be suitable for the estimation of these drugs in bulk and their formulations. Several parameters such as linearity, accuracy, and precision, Limit of detection and Limit of quantification were considered for the analysis of results of the drugs and their corresponding validation were carried out statistically.

Keywords: Chloramine-T, Indigo caramine dye, Spectrophotometry, Drugs.


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