Abstract

OPTIMIZATION OF INCUBATION TIME IN THE SYNTHESIS OF APIGENIN COMPOUNDS WITH RADIONUCLIDE TECHNETIUM-99M AS POTENTIAL RADICAL SCAVENGING AGENT

Danni Ramdhani*, Maula Eka Sriyani, Marisa Dwi Ariani, Eva Maria Widyasari

ABSTRACT

Objective: The purpose of this study is to determine the optimum incubation time for the synthesis reaction between Apigenin flavonoid compounds and Technetium-99m radionuclide compounds. The compound formed is expected to become a potential radical scavenger diagnostic agent. Methods: The method used to optimize the 99mTc-Apigenin compound synthesis reaction process is the determination of the incubation time parameters. The optimum incubation time is determined by making 5 incubation time variations, and the optimum incubation time value is determined by the best radiochemical purity value. Results: The results of optimization of incubation time on the synthesis of 99mTc-Apigenin compound obtained the optimum incubation time for 30 minutes with the highest radiochemical purity value of 86.95% ± 0.35%. Conclusion: The optimum incubation time required for the 99mTc-Apigenin compound formation reaction is 30 minutes, with the least amount of 99mTcO4- and TcO2 impurities at 9.93% ± 2.22% and 3.13% ± 2.46%.

Keywords: Apigenin, incubation time, Technetium-99m, Radiochemical Purity, 99mTc-Apigenin, Radiotracer.