Abstract

CYTOTOXIC ACTIVITY OF COUMARIN DERIVATIVE ON VERO AND HELA CELL LINES

Erwelly B. Oliveira∗, João S.B. Luz, Gleyka D.M. Santos, José Maria Barbosa-Filho, Rodrigo S.A. Araújo, Cláudio G. Rodrigues, Dijanah C. Machado, Eliete C. Silva and Paloma L. Medeiros

ABSTRACT

Natural products have been considered good tools for prospecting of new active drugs or models for new therapeutic drugs. Coumarins are a group of natural phenolic compounds that shows several pharmacological activities and the 3-hydroxycoumarin represents as target valuable molecules against several diseases. The aim of present work was to investigate the cytotoxic activity of 3-hydroxycoumarin on Vero and HeLa cells and its action on the morphology of these cells line. Cytotoxicity was measured using MTT assay and the morphological features were evaluated by phase-contrast microscopy. Significant toxicity was determined for both cell lines and morphological changes were observed from 100 μg/mL. Further studies are needed to investigate other activities of this coumarin and determine potential applications.

Keywords: 3-hydroxycoumarin, cytotoxic activity, HeLa cells, Vero cells, morphological analysis.