Abstract

ANTIPLASMODIAL EFFECT OF HOSLUNDIA OPPOSITA VAHL (LAMIACEAE) LEAVES EXTRACTS

Obou Constantin Okou*, Abba Pacôme Obouayéba, Yapi Guillaume Yayé, Eve Victoire Akpoué and Allico Joseph Djaman

ABSTRACT

The objective of this work was to evaluate in vitro the chemosensitivity of aqueous and hydroethanolic extracts of Hoslundia opposita (Lamiaceae) leaves on Plasmodium falciparum by maturation and ex vivo RSA tests. The maturation test of the different extracts on the five clinical isolates studied showed antimalarial activity, with a promising effect of the aqueous extract (IC50 = 12.66 μg/mL). While the two extracts obtained by hydroethanolic partition have a moderate effect with IC50s between 15 and 50 μg/mL. The results of the ex vivo RSA test confirmed those of the maturation test with a survival percentage of approximately 50% parasites (i.e. 50% inhibition), when the parasites were exposed to the IC50 of the various extracts used during the maturation test. This last test thus shows the effectiveness of the extracts on the young forms (rings) of the parasite. These results make Hoslundia opposita a potential resource in the search for antimalarial molecules.

Keywords: Extracts, H. opposita, antiplasmodial effect, P. falciparum.