Abstract

ISOLATION AND EXTRACTION OF AMYLASE ENZYME FROM AMYLASE PRODUCING BACTERIA ISOLATED FROM SOIL

Sudeep H. Patil and Annika Durve Gupta*

ABSTRACT

Amylases are amongst the most significant enzymes. Such enzymes hydrolyze the starch molecules into polymers composed of glucose units. They also play an important role in Biotechnological studies. In this study, Amylase enzyme producing bacteria were enriched and isolated on Starch agar medium from different soil samples obtained from the area near Thane and Kalyan region. 18 morphologically different bacterial isolates were obtained, out of which, 5 isolates were selected based on the clear zones obtained in the starch hydrolysis test. These five isolates were studied for their Gram nature and colony characters. They were identified using VITEK-2 system at NUCLEUS diagnostic center, Kalyan. The crude enzyme was extracted and purified using Ammonium sulphate precipitation, dialysis and Ion-Exchange Chromatography. The various parameters like effect of pH, temperature, etc on enzyme activity were estimated. Amylases have potential application in a wide number of industrial processes such as food, fermentation, textile, paper, detergent, and pharmaceutical industries.

Keywords: Amylase enzyme, DNSA, Ion-exchange chromatography, Ammonium sulphate precipitation, Zymography.