Abstract

CYTOTOXIC ACTIVITY OF DIFFERENT EXTRACTS FROM LEAVES OF A SPECIFIC MEDICINAL PLANT (SENNA OBTUSIFOLIA)

Mohammed Yousuf*, Md. Rafiqul Islam Rupam, Sadid Uz Zaman, Sharmin Sultana, Md. Imran Nur Manik and Prof. Dr. Md. Harun Ar Rashid

ABSTRACT

This dissertation describes the isolation and characterization of secondary metabolites from the leaves of Senna obtusifolia, a member of Fabaceae family as well as studies of different bioactivities of the crude methanolic extract and its different partitionates i.e. petroleum ether (PEP), dichloromethane (DCM), ethyl acetate (EtOAc) and aqueous (AQP) soluble fractions of leaves of S. obtusifolia. The concentrated methanolic extract of this plant was then subjected to Kupchan portioning protocol to yield different fractions i.e. petroleum ether (PEP), dichloromethane (DCM), ethyl acetate (EtOAc) and aqueous (AQP) soluble fractions. In addition, column chromatography separation and purification of the pet ether soluble partitionate of leaves of S. obtusifolia was done. The methanol extract of S. obtusifolia (ME) leaves and its different partitionates i.e. petroleum ether (PEP), carbon tetrachloride (CTP), chloroform (CHP) and aqueous (AQP) soluble fractions were subjected to brine shrimp lethality to identify the potential source of cytotoxic compounds. The highest lethality revealed by AQSF having LC50 value of 1.80 g/ml followed by PEP (1.52g/ml) in brine shrimp lethality bioassay of different fractionates of the leaves of S. obtusifolia.

Keywords: Extract, Extraction, Zone of inhibition, Antimicrobial activities, Partitionate, Fabaceae.