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Abstract

DETECTION OF EBV IN BLOOD AND SALIVA SAMPLES OF PATIENTS WITH LYMPH PROLIFERATIVE DISORDER

Dr. Hadeel K. Mohammed*, Dr. Zahraa J. Jameel and Dr. Salah S. Mohammed

ABSTRACT

Epstein Barr virus (EBV) is also known as human herpesvirus 4, Is the cause of the disease infection mononucleosis, the virus is associated with many malignant tumors such as lymphoproliferative diseases. This study was conducted in Diyala Governorate at Al Batul Teaching Hospital for woman and Children from October 2018 until May 2019. 30 Samples (Blood and saliva) were collected from children with lymph proliferative disorder, the ages ranged from (1month to 12 years). And 20 samples (Blood and saliva) from apparently healthy children. Blood and saliva samples used for Molecular Test, and serum samples used for ELISA test, The results of blood samples showed that the positive rate of the EBV using the RT-PCR was 2 /30 (6.7%), while the control group of healthy people did not appear to be positive. The age group (47-96 months) recorded the highest positive rate of 10%, The results of the current study showed that the percentage of female positive is higher than that of males, (16.7%) in females, while in males (0%), The saliva samples were positive 9/30 (30%)for the group of patients and 4/20 (20%) for the control group. The age group (97-144 months) recorded the highest positive rate of 40%, The results of this study showed that the percentage of positive male patients was lower than that of females, (27.8%) in males and (33.3%) in females. The ELISA results showed that the highest positive rate of the virus in the case of Reactivated (26%).

Keywords: Epstein-Barr virus (EBV), lymph proliferative disorder, RT-PCR, ELISA.


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