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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: APRIL ISSUE PUBLISHED

April Issue has been successfully launched on 1 April 2024.

Abstract

ANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF RITONAVIR AND LOPINAVIR IN TABLET DOSAGE FORMS BY RP – HPLC

Sunki Praveen Reddy* and Dr. M. Srinivas

ABSTRACT

A simple accurate and precise analytical method was developed for the simultaneous estimation of Ritonavir and Lopinavir in its tablet dosage form by RP-HPLC. This both combination drug has anti-retroviral agents and hence is used for the therapy of human immunodeficiency virus infection in adults and pediatric patients. Mobile phase with degassed methanol: potassium dihydrogen phosphate (KH2PO4) buffer 55:45V/V was run through standard discovery, Inertsil C18 column(4.6x150mm,5μm) column at a rate of 1ml/min at 25°C and optimized wavelength was 275nm.Retention time for Ritonavir and Lopinavir was 2.997min and 4.370min, %RSD of 0.2% and %recovery of 0.6% were obtained for Ritonavir and Lopinavir respectively. LOD and LOQ values obtained for Ritonavir and Lopinavir. All parameters of validation are found inside the range and method was precise and reliable which is also economical that can be adopted in regular quality control test in Industries.

Keywords: Lopinavir, Ritonavir, RP-HPLC.

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