Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ISONIAZID AND RIFAMPICIN IN TABLET DOSAGE FORMS BY RP-HPLC

P. R. Logesh Kumar*, Raj Purohit Kamala, Valleti Divya, D. N. Sree Vidya, Midde Saileela, Ganta Swetha Kumari, Kooni Navaneetha

ABSTRACT

Pharmaceutical analysis is a branch of practical chemistry that involves a series of process for identification, determination, quantification and purification of a substance, separation of the components of a solution or mixture, or determination of structure of chemical compounds. The substance may be a single compound or a mixture of compounds and it may be in any of the dosage form. The substance used as pharmaceuticals are animals, plants, micro organisms, minerals and various synthetic products. RPC refers to liquid (rather than gas) chromatography. The term "reversed-phase" has a historical background. As a result, hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydrophilic molecules in the mobile phase will pass through the column and are eluted first. Rifampicin inhibits bacterial DNA-dependent RNA synthesis by inhibiting bacterial DNA-dependent RNA polymerase. Crystal structure data and biochemical data suggest that rifampicin binds to the pocket of the RNA polymerase β subunit within the DNA/RNA channel, but away from the active site.

Keywords: Rifampicin, INH, Analysis, spectral analysis.