Abstract

SILVER NANOPARTICLES CONJUGATED WITH CIPROFLOXACIN HCL TO COMBAT BACTERIAL- RESISTANCE VIA TOPICAL DRUG DELIVERY SYSTEM

Mohit Sharma* and Rahul Sharma

ABSTRACT

Gradual expansion of the development of antibiotic resistant in bacteria is cause of severe global threat. Innovative methods are now being developed to control the microbial infections in order to reduce the mortality along with infection rates. Combination of antibiotics and metallic nanoparticles are effective against multi drug resistant microorganisms. Nanoparticles (NPs) are reported to show antimicrobial activity. Herein, describe successful synthesis of ciprofloxacin HCl loaded silver nanoparticles (AgNPs) by co-precipitation method and their incorporation in the gel base of Carbapol 940. Successful synthesis of silver nanoparticles and their conjugation with ciprofloxacin HCl was confirmed by various test (zeta poteinal, particle size measurement, silver content, drug loading and entrapment efficiency). AgNPs were small in size with particle size distribution as obtained from TEM. To determine the release mechanism of formulated gel various models such as zero-order, first-order, Higuchi and Korsmeyer-Peppas were fitted to the release data. The enhancement in antimicrobial effect of drug was seen when combined with silver nanoparticles. Synergistic and additive effects were found with activity of ciprofloxacin HCl when doped with silver nanoparticles with in a gel base against S. aurease and MRSA During evaluation of minimum inhibitory concentration (MIC) values, similar concentration of prepared gel was capable of producing antibacterial activity.

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