Abstract

THE EFFECT OF PROLONGED ADMINISTRATION OF AQUEOUS EXTRACT OF THE STEM BARK OF KHAYA SENEGALENSES ON THE LIVER AND KIDNEY OF WISTER RATS

Uchegbu Anurika Udochukwu,Obeagu Emmanue Ifeanyi*,Odo Matthew Chibunna, Emelike Chinedum Uche

ABSTRACT

Khaya senegalensis is a medicinal plant that has been used for the treatment of different ailments. Study has shown that it has been reported that increased dosage and prolonged administration of Khaya senegalensis induces significant changes on some biochemical parameters. This work was therefore designed to study the effect of prolonged dosage of 50m/kg body weight. The effects on some biochemical parameters were investigated. The wister rats were randomly divided into 7 groups of 5 rats each: the control group, and soups A,B,C,D,E and F in which each of the group was administered with the extract for 24hrs, 5days, 10days, 20days and 25days respectively. Blood samples were collected and ALT (Alanine Transaminases), AST (Aspartate transaminases), ALP (Alkaline phosphatase) and GGT (Alanine transaminases), plasma levels investigated using Enzymatic method. Na+ and K+ plasma levels were assayed using flame photometric method. C1- and HCO3 - was investigated using Tritrimetric method while creatinine plasma level was investigated using spectrophotometric method. Administration of the extract resulted to no change in the serum aspartate transaminase activity after administration of the extract in all the (24hrs, 5days, 10days, 15days, 20days, 25days while significant reduction (p<0.5) occurred only in group D (after 15 days administration) of ALT. administration of the extract also led to a significantly increased (P<0.05) only in group F (after 25 days) when compared with the control. There were no changes in most of the electrolytes (serum K+, HCO3 - and CI-) in all the groups while significant reduction (P<0.05) began in plasma Na+ after 10 days. Furthermore, there was significant reduction (P<0.05) in plasma creatine level in groups after 5 days, 10 days and 15 days, In conclusion, no change (P<0.05) in mean plasma AST and significant reduction P<0.05) in ALT suggest no hepatocellular damage.

Keywords: Khaya senegalensis,liver function,kidney function.