EVALUATION OF CARDIOPROTECTIVE ACTIVITY OF POLYALTHIA LONGIFOLIA AGAINST DOXORUBICIN INDUCED SUB ACUTE CARDIOTOXICITY IN RATS
Rajashekar Perusomula* and Ramesh Alluri
ABSTRACT
The cardio toxicity of doxorubicin (DOX) has limited its use as an anticancer drug. The Objective of present study was to investigate the cardio protective effect of Polyalthia longifolia var (Annonaceae) (PL) against the cardio toxicity of Doxorubicin (DOX) in rats. Materials and Methods: Male Wistar rats (200-250 g) were randomly divided into four groups. Group I was vehicle control. Group II animals received Doxorubicin 4 mg/kg i.p. on days 7, 14, 21 and 28. Group III and IV animals were treated with methanol extract of Polyalthia longifolia (PL) (300 and 600 mg/kg, respectively) for 30 days. Doxorubicin was administered on days 7, 14, 21 and 28. After anesthetizing the animals on the 30th day Electrocardiogram (ECG), was recorded and blood was investigated for Creatine Kinase-MB is enzyme (CK-MB), Lactate Dehydrogenase (LDH) and Aspartate
Aminotransferase (AST) while determination of Superoxide Dismutase (SOD), Reduced Glutathione (GSH), Lipid Peroxidation (LPO). At the end of the study, the histology of heart was carried out. Results: Group 3 and 4 of animals showed decreased QT and ST interval (p<0.01) and was non significant in heart rate compared to Group 2. Significant decrease in serum CK-MB, AST and LDH of group 3 (p<0.01) and group 4(p<0.001) of animals was observed as compared to group II. In group 4 significant increase in the level of GSH (p<0.01) and decrease in MDA content (p<0.01) was observed as compared to group 2. Histopathological study 600mg/kg treated group showed protection against myocardial toxicity induced by Doxorubicin. Conclusion: It is concluded that Polyalthia longifolia Var possessed moderate cardio protective effect at dose 600mg/kg against Doxorubicin induced cardio toxicity in rats.
Keywords: Antioxidant, electrocardiogram, histopathology, cardiotoxicity, tissue parameters.
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