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Abstract

METHOD DEVELOPMENT AND VALIDATION FOR AMLODIPINE BESYLATE AND CARVEDILOL IN COMBINE PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC

P. Prashanthi* and M. Srinivas

ABSTRACT

A new method was established for simultaneous estimation of a Amlodipine Besylate and Carvedilol by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Amlodipine Besylate and Carvedilol by using Inertsil -ODS C18(250 x 4.6 mm, 5μ), flow is 1.0 ml/min, mobile phase ratio was Phosphate buffer and Methanol (30:70), detection wave length was 254 nm. Chromatogram observed at RT’s of 3.661min to Amlodipine Besylate and 6.453min to Carvedilol.

Keywords: Amlodipine Besylate and Carvedilol, RP-HPLC, Acetonitrile.


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