Abstract

LARVICIDAL EFFICIENCY OF PIPER NIGRUM L. AND CARICA PAPAYA SEED EXTRACTS ON AEDES AEGYPTI THIRD INSTAR LARVAE

Glen Mangali*, Roselle Habana, Jeannette Sabocojan and Rosette Taguba

ABSTRACT

Worldwide, dengue haemorrhagic fever is a major health concern, particularly in Southeast Asia because of its environment. In the Philippines, local plants are being studied for mosquito control potentials. The present investigation involved the laboratory study of larvicidal efficiency of ethanolic extracts of dried seeds of both Carica papaya and Piper nigrum combined with varied concentrations against of Aedes aegypti, the vector of dengue. When analyzed individually, the plant extract combination with 70% Piper nigrum and 30% Carica papaya concentration content yielded the highest mortality rate against third instar larvae of Aedes aegypti followed by 50% Piper nigrum and 50% Carica papaya concentration and 30% Piper nigrum and 70% Carica papaya concentration with the least larval kill. The LC50 values of ethanolic extract of 70% Piper nigrum and 30% Carica papaya concentration, 50% Piper nigrum and 50% Carica papaya concentration and 30% Piper nigrum and 70% Carica papaya concentration against third instar larvae are 9,032.85, 12,253.05 and 14,587.42 mg/L, respectively. The LC90 values are 28,590.1, 30,421.75, 32,215.47 mg/L, respectively. Toxicity of ethanolic extracts of dried Carica papaya and Piper nigrum provided an excellent potential for controlling Aedes aegypti at the larval stage. Performing individual larvicidal testing of Piper nigrum and Carica papaya seeds for further comparison of data is recommended.

Keywords: Aedes aegypti, Piper nigrum, Carica papaya seed, crude extracts, larvicide.