Abstract

DETERMINATION OF ANTIOXIDANT CAPABILITY OF N-(1-NAPHTHYL) ETHOXYBENZOHYDROXAMIC ACID

Priyanka Singh and Rama Pande*

ABSTRACT

The antioxidant capacity of N-(1-naphthyl) ethoxybenzo hydroxamic acid (NEBHA) has been determined through 2,2'-Diphenyl-1- picrylhydrazyl free radical (DPPH) and lipid peroxidation inhibition methods. It caused 50 % inhibition of DPPH concentration (EC50) at 389.51 mM. Its lipid peroxidation inhibition ability was 43.78 % at 389.51 mM. Both the methods confirm that NEBHA is capable of inhibiting the free radical mediated DNA damage. The antioxidant potential of NEBHA was assessed by gel electrophoresis method as well. This method confirmed the DNA cleavage protection ability of the compound against H2O2-mediated DNA damage.

Keywords: DNA cleavage protection, Free radical scavenging activity, N-(1-naphthyl) ethoxybenzo hydroxamic acid.