Abstract

NEW REAGENT FOR SPECTROPHOTOMETRIC DETERMINATION OF METFORMIN IN PURE FORMS AND IN PHARMACEUTICAL FORMULATIONS

Malek Okdeh* and Mohammad Obid

ABSTRACT

An accurate, simple, fast and cheap spectrophotometric method has been developed for the determination of Metformin in pharmaceutical pure and dosage forms The method is based on the formation of violel complexe between metformin and chrome azurol S in phosphate buffer at pH=2.5. The resulting complex was measured at λmax=570 nm. The effect of variables such as reagent concentration, and metformin concentration investigated to optimize the procedure. Beer’s law was obeyed in concentration 3.31-57.96 μg/ml and the defection limits LOD and quantitation (LOQ) for proposed method are 0.781 and 2.660 μg/ml respectively, Recovary studies and statistical data proved the accuraty reproducbility of the proposed method. The common excipients did not inteffer in this analysis. Hence the methed is useful for routine estimtion of pure metformin and in pharmaceutical formulations.

Keywords: Metformin, chrome azuroL S, complex formation, Spectrophotometric determination.