Abstract

EFFECT OF CITRUS LIMON JUICE AND TAMOXIFEN ON THE OXIDATIVE STRESS ACTIVITIES OF MCF-7 CELL INDUCED BREAST CANCER IN SPRAWGUE DAWLEY RATS

Samson Adewale Oyebadejo*, Oyepata Simeon Joseph, Samson Oluwaseyi Adesite and Emem Rapheal Abia

ABSTRACT

In search of possible, safe and toxic anticancer drug for the treatment of breast cancer, molecular pathological study of the activities of Citrus limon juice on MCF-7 cell line induced breast cancer in Sprague Dawley rats compared to Tamoxifen, Breast cancer reference drug was carried out, over one hundred and twenty Sprague dawley rats of 40 days old, average body weight 180-220g were divided into ten (10) containing of 12 animals per group, group 1 was control, fed only with rat chow and water, group 2 was MCF-7 cell line induced rat alone (BCIR only), group 3 Citrus limonj uice (CLJ) at 8.88%, Group 4 Citrus limon juice(CLJ) at 17.32%, group 5 Citrus limon juice(CLJ) at 25.98%, group 6 was given 0.2mg/kg of Tamoxifen alone, group 7 (BCIR+CLJ at 8.88%), group 8 (BCIR+CLJ at 17.32%), group 9 (BCIR+CLJ at 25.98%) and group (BCIR+ 0.2mg/kg of Tamoxifen). Acute and sub – acute toxicity were carried out after the establishment of safety dose following determination of LD50, at the end of the administration of the Citrus limon juice and Tamoxifen, animal were sacrificed, fresh blood were taken into plain bottles without anticoagulants, serum was collected and stored at 8oc for Biochemical investigation of Oxidative stress activities; TAC, LDH, SOD and LPD (MDA) were carried out, Result, Oxidative stress activities showed significant increase in TAC, LDH, SOD and LPD (MDA) at P>0.001 in BCIR when compared to the BCIR+CLJ, BCIR+Tamoxifen and non-BCIR groups. In conclusion, this study showed that Citrus limon juice posses strong anitioxidative capacities with potential anticancer activities and does not pose pathological conditions on the body during and after usage.

Keywords: MCF-7 Cell lines, Breast cancer, Citrus limon Juice, Tamoxifen, Molecular Pathology, Sprawgue Dawley Rats.