EMERGENCE OF PANTOEA AGGLOMERANS, CO-PRODUCING BLAOXA-48 AND BLANDM-1 CARBAPENEMASES, AT A TERTIARY CARE CENTRE AT CENTRAL INDIA
Atul Khajuria*, PhD Scholar and Ashok Kumar Praharaj, MD, PhD Microbiology
ABSTRACT
Objective: The aim of this study is to evaluate the expression of metallobetalactamase genes in P.agglomerans recovered from hospitalized patients in a tertiary care hospital. Materials and Methods: A total of 22 P.agglomerans all non-duplicate clinical isolates were recovered from clinical specimens of hospitalized patients admitted to the Medical and Surgical intensive care units Carbapenem resistant strains were isolated and presence of the metallo carbapenemase enzyme was confirmed by Polymerase chain reaction (PCR) assays and sequencing. Transferability of genes was determined
by conjugation experiments. REP PCR, ERIC PCR and RAPD PCR assays carried out to check Isolate relatedness. Results: Out of 22 isolates, 17 were found to be carbapenem resistant as MICs was >4μg/ml against IPM, MEM, and ETP as determined by the E-test and VITEK-2. Among the tested genes, blaNDM-1 was the most prevalent gene as it was detected in 17, bla VIM was not detected, blaCTX-M-15, and blaCTX-M-14 are the commonest CTX-M ESBLs that were present in 15, and 2 isolates. blaSHV-5, blaSHV-11, blaSHV-12, and blaSHV-28 are the commonest SHV genes detected. Conclusion: This study provides an insight into the acquisition and emergence of blaOXA-48 & blaNDM-1 producing P.agglomerans, and emphasizes its transmission capability through plasmids.
Keywords: blaOXA-48, blaNDM-1, blaSHV-5, blaSHV-11, blaSHV-12, blaSHV-28, blaCTX-M-15, blaCTX-M-14, REP PCR, and RAPD PCR.
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