Abstract

EVALUATION OF ANTI OSTEOARTHRITIC ACTIVITY OF CHIA SEEDS (SALVIA HISPANICA) IN RATS

Arpitha M.*, Dr. M. S. Rajesh, Devika Rani K. and Dr. S. Ramachandra Setty

ABSTRACT

Osteoarthritis(OA) is a classic age-related chronic degenerative disorder. In OA degradation and loss of the articular cartilage is a central feature that is sometimes attributed to “wear and tear”. The present study was designed to evaluate the anti-osteoarthritic activity of Salvia hispanica. Seeds of Salvia hispanica were extracted with mixture of water and ethanol (30:70). Invivo anti-inflammatory activity and anti-osteoarthritic activity were evaluated. Acute toxicity studies were carried out as per OECD guideline 420. The effect of hydro alcoholic extract of seeds of Salvia hispanica were screened using models like Corticosteroid induced OA for anti-osteoarthritis, Carrageenan induced paw edema (1% carrageenan) and Cotton pellet method, by implanting 10mg of cotton subcutaneously for anti-inflammatory activity. Diclofenac sodium (11mg/kg b.w) and alandronate sodium (1mg/kg b.w) were used as reference standard, the degree of protection was determined by bone density, bone width, serum calcium, and phosphorous for anti-osteoarthritis and granuloma tissue formation and paw volume were evaluated for anti-inflammatory effect. Treatment with hydro alcoholic extract of chia seeds significantly increased serum calcium, phosphorous and bone density favouring anti-osteoarthritic activity, and it also exhibited inhibition in granuloma tissue formation and decrease in paw volume facilitating anti-inflammatory activity. Further the histopathological evaluation revealed decrease in parameters like bone resorption, cyst formation and depth surface induced by corticosteroids which may be helpful in preventing osteoarthritis and inflammation respectively.

Keywords: Osteoarthritis, Salvia Hispanica, Anti-Inflammatory.