Abstract

DETERMINATION OF MEDIUM LETHAL DOSE (LD50 VALUE) FOR ORAL ACUTE TOXICITY OF ROYAL JELLY

Syeda Hina Kausar* and Dr. Vitthal Ram More

ABSTRACT

Objective: RJ a honey bee product has a high medicinal values to treat various diseases such as cancer, inflammation, various skin problems etc. current study was designed for the determination of oral medium lethal dose (LD50 value) of RJ. Material and Method: The study was directed according to OECD 423 guidelines by using male Swiss albino mice weighing 20-25 gms. all were divided into six groups. Group G1 was served as control group which received normal saline (NS) while group G2 to G6 were orally treated with single daily dose of 100, 500, 1000, 2000 and 5000 mg/kg of RJ, respectively. All the animals were closely observed for any sign of toxicity illness and behavioral changes. Results: Obtained results demonstrated that no mortality was observed in all treatment groups for post 24 hours but the dose 2000mg/kg show mortality at 14th day while high dose up to 5000 mg/kg exhibited mortality at day 7 and 14 days observation duration. Conclusion: In conclusion, acute exposure of RJ up to 2000 mg/kg was safe in male mice without causing any adverse effects or mortality. The oral LD50 of Rj suggested to be greater than 1616 mg/kg in male mice.

Keywords: Royal Jelly, LD50 Value, Acute Oral Toxicity, Swiss Albino Mice, Mortality.