Abstract

GREEN SYNTHESIS AND CHARACTERIZATION OF COPPER NANOPARTICLES USING CARICA PAPAYA LEAF EXTRACT AND SCREENING ITS ANTIBACTERIAL EFFICACY

Ajay Kumar Sahu, Rahul Nemani*, Dr. Rupali Sinha, Deepthi Sandeep and Shobha M.

ABSTRACT

Nanotechnology in recent trend is becoming a boon to mankind. Its demand in the commercial market is increasing now a day because of its wide applicability in research fields and commercial market equipments like electronics, catalysts, medicine, microchips and energy. Most of these nanoparticles which are synthesized now a day is metallic and it can be synthesized y wet chemical techniques. This research work mainly focuses on the cost effective synthesis of copper nanoparticles in a eco-friendly technique through the extract of carica papaya plant leaves from 1mM solution of Copper Chloride mixed uniformly with L-Ascorbic Acid which acts a anti-oxidant agent and NaOH which acts a catalyst to catalyze the reaction and Hydrazine Hydrate which acts as a reducing agent for the chemical reaction to occur. Plant samples were collected from Baiyappanhalli in Bangalore,INDIA.After the plant extract was collected synthesis of nanoparticles were done which was then further send for U.V spectroscopy analysis and TEM results for the characterization of these copper nanoparticles.Then further its antiaacterial efficacy was screened against Bacillus and E.coli species using standard disc diffusion method.

Keywords: Carica Papaya leaf extract, Copper nanoparticles, green synthesis, U.V spectroscopy, TEM scanning, antibacterial efficacy.