Abstract

SEASONAL EFFECT ON EXTRACTION, QUANTITATION & PURITY ESTIMATION OF GENOMIC DNA FROM SYZYGIUM CUMINI LINN. LEAVES

Prince Ojha, Buddhajyoti Ghosh, Tanaya Ghosh, Prasenjit Mitra and Prasanta Kumar Mitra*

ABSTRACT

Syzygium cumini Linn. (S. cumini L.), is an important plant having several medicinal values. In the present investigation attempts were made to isolate pure DNA from S. cumini L. leaves with the objective that extraction of DNA from the plant leaves will help the genotype analysis leading to scientific identification of the plant as well as plant breeding and genetic diversity. Attempts were also made to study the seasonal effect on quantity of pure DNA from S. cumini L. leaves as, it is known, that season has effect on amount of chemicals in the plant parts. Leaves of S. cumini L. were collected during autumn, winter, summer and rainy seasons, identified by experts and processed for extraction, quantitation & purity estimation of genomic DNA by the standard methods. Results showed that pure DNA was extracted from S. cumini L. leaves in high amount during rainy seasons (June – August). It is concluded that S. cumini L. leaves of rainy season may be used to get high amount of pure DNA.

Keywords: Syzygium Cumini Linn, DNA Extraction, Purity Estimation, Effect of Season.