Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF FLUOCINOLONE ACETONIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Shubhangi Sidram Bamgonde*, Dr. Mallinath Shankarappa Kalshetti, Zeba Mahamad Hanif Gaibu, Neha Ranjeet Gate, Kaveri Chandrakant Dulange

ABSTRACT

A rapid, simple, selective, sensitive, precise and specific UV Spectrophotometric method has been developed for the determination of Fluocinolone acetonide in bulk and (200-400nm) in 1cm quartz cell in a double beam UV Spectrophotometer. The spectrophotometric detection was carried out at an absorption maximum of 235 nm using Methanol as solvent. The detector response for the Fluocinolone acetonide was linear over the selected concentration range 1.25-6.25 μg /ml with a correlation coefficient of 0.999 and equation for the regression curve was found to be y=0.1596x + 0.0175. The accuracy was between 99-100 %. The precision (%RSD) among six samples preparation was 0.121 %. The LOD and LOQ was 0.1125 And 0.309 μg /ml respectively. Statistical analysis proved that the methods are repeatable and specific for the determination of the said drug. These methods can be adopted in the routine assay analysis of Fluocinolone acetonide in bulk and pharmaceutical dosage form.

Keywords: Fluocinolone acetonide, UV Spectrophotometer, Methanol, Method Validation.