Abstract

IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF LEAF EXTRACT OF PASSIFLORA LIGULARIS

*M.Razia, V.Karithga, W.Bernala, L.Paul Evangelin Shavisha, S.H.Saira Banu

ABSTRACT

Passiflora ligularis is a medicinal plant belonging to the family Passifloraceae with several ethnomedicinal properties. In this study, leaf P. ligularis were screened for the presence of major phytochemical groups. Phytochemical screening of the leaf of P. ligularis showed the presence of alkaloids, flavonoids, terpenoids, steroids, phenolic compounds, glycosides and cardiac glycosides. The total phenolic content was measured by Folin-Ciocalteau was found to be 27 mg GAE/ g. The antioxidant capacity of methanol extract was investigated based on their ability to scavenge (DPPH) stable free radical. In the DPPH assay, the IC50 value was found to be 48μg/ml. The antibacterial activity of P. ligularis was tested against six pathogenic bacteria Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Shigella flexneri and Salmonella typhimurium. The methanol extract of P. ligularis (100 mg/ml) showed maximum zone of inhibition (16mm) against P. aeruginosa, E. coli. S. flexneri showed less zone of inhibition (12mm). The aqueous extract (100 mg/ml) showed maximum zone of inhibition (11mm) against E. coli. The antimicrobial activity revealed that methanol extract showed significant activity than the aqueous extract.

Keywords: Passiflora ligularis, Phytochemical, Antioxidant, Antimicrobial activity.