Abstract

COMPARATIVE ANALYSIS OF BENDAMUSTINE HYDROCHLORIDE CYTOTOXICITY IN HEALTHY HUMAN LYMPHOCYTES AND L5178Y TK+/- MOUSE LYMPHOMA CELLS

Pasula Chandra Sekhar*, Dr. Savithiri Shivakumar,Tadakaluru Yasodha Lakshmi, Sannidhi Ranga Suresh

ABSTRACT

The study was conducted to evaluate and compare the cytotoxicity of Bendamustine hydrochloride in Mammalian Chromosome Aberration Test (CAT) In vitro, using cultured healthy human whole blood lymphocytes and In vitro Cell Gene Mutation Assay (CGM) using L5178Y TK+/- mouse lymphoma cells as per OECD guidelines No. 473 & 476 [1,2] respectively, both in the presence (2%v/v) and absence of metabolic activation system up to the cytotoxicity. Bendamustine hydrochloride is cytotoxic at the doses 300, 150, 75, 37.5, 18.75, 9.37 μg/ml of culture medium and non-cytotoxic at 4.68 μg/ml in CAT where 300, 150, 75, 37.5μg/ml of doses are cytotoxic and 18.75, 9.37, 4.68μg/ml doses are non-cytotoxic in CGM, both in the presence and absence of metabolic activation system. Comparatively Bendamustine hydrochloride test doses are more cytotoxic in the presence of metabolic activation system (2%v/v) than absence both in CGM, CAT and lower doses of Bendamustine hydrochloride is cytotoxic in CAT when compare with CGM.

Keywords: Bendamustine hydrochloride, Cytotoxicity, metabolic activation.