Abstract

BACTERIOLOGICAL STUDY OF AIR QUALITY IN THE OPERATING THEATERS IN DIYALA GOVERNORATE \ IRAQ

*Muthanna A. Al-Mahdawi, Namariq M. noman and Mohamed D. Al Mashhadani

ABSTRACT

The study aims at assessing the bacterial load in the air and its relationship with the pollution control program in the operating halls of Baquba Teaching Hospital, Governorate of Diyala / Iraq. a total of 387 samples were collected from December to March 2018, including 270 samples of microbial load of operational halls air (first, second and third surgical hall, main corridor and sub-corridor leading to halls). The samples were collected twice daily as the began of the work in the hall at (8.00 AM 135 samples) and (at midday at 12.00 AM135 samples) by the Active Sampling method, Also 135 swabs were taken within the routine work of the pollution control panels before starting work from (five sites walls, patient beds, anesthesia trolley and fluid retrieval system). The results of microbial culturing of the microbial air load samples showed positive culture in 90% of the plates (270 plates) gave positive results. The colony forming unite ranged from 0-213 CFU and the number of colony-forming units per cubic meter in the air was between 0 - 280 CFU\m3. The level of microbial load varies according to the time of the air examination and location, as the daily microbial load rated at morning (21.79, 15.44, 30.16, 27.12 and 27.67) for the first, second and third surgical halls and the main and sub- corridor leading respectively and at midday (58.96, 46.07, 72.25, 77.44 and 61.57) respectively. These differences were statistically significant at P level <0.0 5. It gave positive germination dishes to the air samples at the start of work 132 isolation at isolation rate (20.74%) and positive germination dishes at afternoon 168 isolation at isolation rate (62.22%), while the percentage of swabs for pollution control was 20.74% (28/35). The results of bacterial isolation diagnosis from the microbial load of the operations halls showed that the bacteria Staphylococcus which positive for gram stain accounted for 54% of the isolation aggregate and were distributed to the Staph auras 92 isolation (30.66%), followed by Staph epidermis 70 isolation (23.33%). While the negative bacillus to gram stain formed a percentage of 46%, Pseudomonas.ssp was the most common among the isolation, 46 isolation (15.33%) followed by Enterobacter.ssp (42% isolation), E.coli 40 isolation (13.33%) and protuse.ssp 10 isolation (3.33%). In pollution control swabs, the most common bacteria were Staph epidermis 18 isolation (72%) and Bacillus.ssp 4 isolation (16%) and E. coli 3 isolation (12%). The results of the statistical analysis using ROC curves showed that no correlation between bacterial isolation isolated from air and isolation isolates from control swabs (4.43 and 2.5) respectively.

Keywords: Air sampling, SAS air sampling, Airborne Microorganisms, microbial quality, bio aerosols.