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*Falguni B.Tandel1, Dr.Rajesh K.S.1, Nikita P. Patel1, Dr.Khurshid i.Molvi2, Prof. Saurabh S Pandya3.

1Quality Assurance Division, Parul Institute of Pharmacy, Limda, Waghodiya,Vadodara- 391760, Gujarat, India.
2 Ali Allana College of Pharmacy, Akkalkuwa, Nandurbar, Maharashtra.
3B.Pharmacy College, Rampura, Godhra, Gujarat, India.


A newly synthesized anti-inflammatory agent (Methyl 2-(p- Methoxylanilino)-5-(4-methoxybenzoyl)- 4-methylthiophene-3- carboxylate) showed anti-inflammatory activity at a very low dose as compared to both of the standard drugs mefenamic acid and ibuprofen. So for further development of this drug simple, precise, robust and accurate methods has been developed. The drug was estimated by Simple and Derivative UV spectroscopy using Shimadzu UV 1800 spectrophotometer using matched cuvette of 1 cm path length. Simple UV Spectroscopic method is developed at 228nm and Derivative Spectroscopy is developed at 360.6nm/398.6nm in methanol. The method was validated as per ICH Guideline. The linearity-range for the drug was found to be2-10 μg/ml for both the methods. The correlation co-efficient for both simple and derivative spectroscopy is 0.999 proving good linearity between concentration of drug and response for both the methods. The methods are accurate within the range 2-10μg/ml. The % RSD values of repeatability, intermediate precision and reproducibility were less than 2, which indicate that both the methods has good reproducibility. Both the methods are robust and withstands the small but deliberate changes. Thus the proposed methods are simple, accurate and precise and suitable for routine analysis of the drug.

Keywords: Antiinflammatory Agent, Derivative Spectroscopy.

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