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Abstract

APPLICATION OF THREE CHEMOMETRIC METHODS FOR SIMULTANEOUS DETERMINATION OF AMPROLIUM, DIAVERIDINE AND SULPHAQUINOXALINE: A COMPARATIVE STUDY

Fathy M. M. Salama, Khalid A. M. Attia, Ragab A. M. Said and Abdul-Aziz M. M. El-Attar*

ABSTRACT

Three chemometric methods; classical least squares (CLS), concentration residual augmented classical least squares (CRACLS) and spectral residual augmented classical least squares (SRACLS); were investigated. The methods were used for the simultaneous quantitative analysis of amprolium, diaveridine and sulphaquinoxaline in raw material and pharmaceutical dosage form. A 3-factor 5-level experimental design was established resulting in 25 mixtures containing different ratios of amprolium, diaveridine and sulphaquinoxaline. Seventeen mixtures were used as a training set and the other eight were used as a validation set. The results obtained were compared statistically with those obtained from the reported method, viewing no significant difference with respect to their predictability. No organic solvents were utilized during these methods. Hence, they can be considered as remarkable instance for green chemistry.

Keywords: Spectrophotometry; Multivariate calibration; classical least squares; amprolium; diaveridine; sulphaquinoxaline.


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