Abstract

HISTOPATOLOGICAL IMPACT OF MALATHION ON THE TESTICULAR CELLS OF FRESHWATER CRABS BARYTELHUSA CUNICULARIS (WESTWOOD, 1836)

Shipra Sinha*, Gajendra Yadav, Bhawna Rani Kaushik, Satyam Dounde and Devendra Janghel

ABSTRACT

Crabs are particularly useful in aquatic environment and play a very important role in the ecosystem processes and good indicator for polluted condition. The terrestrial crab plays a significant modal in the study of population and also beneficial for human activities. The present study deals with the histochemical changes of organophosphate, malathion in the freshwater crab Barytelphusa cunicularis. Live specimens of Barytelphusa cunicularis were collected from local wet areas of Maroda-sector, Bhilai, (C.G). Average weight of crabs varies from (45-50 gms), the carapace length and carapace width varies from 3.20-4.50mm and 4.25-5.80mm respectively. The crabs were acclimatized in the laboratory condition, and kept in two groups the control group set free from Malathion and the experimental group was exposed to malathion for LC50 at different concentration 0.45ppm, 0.30ppm, 0.26ppm and 0.25ppm for 24hrs, 48hrs, 72hrs and 96hrs respectively. The sections of 5μm were taken for the histopathological examination of testis, the testicular follicular cells interstitials cells and nutritive cells were decreased and distracted number of sperm cells also affected due to the impact of malathion. The result suggests that there is much decrease in the level of sperm cells after exposure, may be due to its rapid utilization to meet the energy demands under the impact of malathion.

Keywords: Freshwater crab, Testis, Histopathology, Pesticides, Malathion.