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Zakaria Hassan Hamid and Rehab Jomaa Mansour*


A factorial experiment with Randomized Complete Block Design was carried out in the plastic house of the Department of biology / College of Science/ University of Diyala for the autumn season 2016 to study the effects of drought stress and Glutamine spraying in total chlorophyll concentration, proline concentration, total carbohydrates and relative damage of the plasma membrane. The experiment was conducted using two levels of stress S1 and S2 and three levels of Glutamine (0, 1000, 2000 mg l-1) with three replicates. The results showed a significant superiority of the first stress level S1 in the mean concentration of chlorophyll recorded 0.66 mg g-1, while the total carbohydrate concentration decreased to 350.6 mg g-1, proline to 1.4 mg g-1 and plasma membrane damage to 52.7% compared with the second stress level S2 which recorded values that amounted 0.56 mg g-1, 460.8 mg g-1, 1.6 mg g-1 and 61.7% respectively. Glutamine showed significant increase in the concentration of total chlorophyll, total carbohydrate and significant decrease in the relative damage of the plasma membrane. The highest concentration of total chlorophyll was recorded in the treatment of spraying with 2000 mg l-1 it amounted to 0.66 mg g-1 while the concentration of total carbohydrates 440.3 mg g-1 also recorded the lowest value of relative damage amounted to 54.6%.Treatment of spraying 0 mg l-1 recorded values amounted to 0.55 mg g-1 and 356.7 mg g-1 and 60.6% respectively

Keywords: Drought stress, Glutamine, Cucumis sativus.

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