Abstract

TOXICITY IMPACT OF SILK DYE WASTE EFFLUENT ON BIOCHEMICAL ESTIMATIONS OF SWISS ALBINO MALE MICE MUS MUSCULUS AND MITIGATION USING MORINGA OLEIFERA LEAF EXTRACT.

Serina Khatun* and Manish Chandra Varma

ABSTRACT

The environmental toxicology studies, the effect of environmental toxicant on the health of organisms and on the different compartments of the environments. Despite the scientific studies carried out over the years, on the toxic effect of the silk dyes waste effluents on organisms. But current study has been premeditated to evaluate the effect of Moringa oleifera (Lam) leaf extract on albino male mice Mus musculas which is subjected to silk dye waste. The biochemical estimations have been taken on account. Five sets of animals i.e. Group I (Control), Group II (fed with 50% silk dye), Group III (fed with 100% silk dye), Group IV (mice fed with 50% dye treated with M. oleifera leaves powder), Group V (mice fed with 100% dye treated with M. oleifera leaves powder) have been taken for experiment. The dose of silk dye was 2ml/day to both groups II and III and M. oleifera leaf is given as per the standard dose (300mg/kg b.w) to both animals of group IV and V. The results show that the M. oleifera leaf extract when fed to Gr IV and V mice, increased significantly the Serum Cholesterol and Seminal Fructose (p<0.05, p<0.10) and when compared with mice of Gr- II and III. Then the M. Oleifera leaf increased significantly number of sperm count and % of sperm motility but decrease the % of sperm mortality of sperm when compared with Gr-II and III at 5% and 10% level of probability. This study suggested that the extract may have beneficial effect on biochemical components such as Serum Cholesterol and Seminal Fructose concentration and sperm count, mortality and motility.

Keywords: Silk dye waste, Moringa oleifera leaf powder, Biochemical estimation, sperm profile, Swiss albino male mice, toxicity assessment.